Regional Website


   Basic Equipment and Materials for Sampling
      1. Motorized boat - technical personnel and boat operator
      2. Station map - showing the different sampling stations
      3.Plankton net - 30 cm in mouth diameter, 100 cm long, 20 micro-meter mesh size and attached
         with a lead (Pub) that weights 1.0 kg, with calibrated rope
      4. Polyethylene bottles - 50 ml - 250 ml capacity, sampling bottles
      5. Accessory kit - masking tape, pencil, waterproof pen, knife and small basin
      6.Slate board and pencil - for data recording, write the format of the "Field Sampling Data Sheet"
      6.Slate board and pencil - for data recording, write the format of the "Field Sampling Data Sheet"
      7.Plastic bags - use to put shellfish samples
      8. Cooler box / Styro box - accommodate water and shellfish samples
      9. Formalin solution - commercial grade (37%)
     10. Sampling equipments - Refractometer, DO Meter (YSI 55) and pH Meter

   Shellfish Collection and Labelling of Samples
      *Methods of Collection
          1. Collect about 20-30 specimens of green mussels and oysters (the number depends on the size, as long as it will give a tissue of about
             100g).
          2. Clean the fresh shellfish by scrubbing the mud, silt and other adhering materials.
          3. Keep the shellfish samples in a plastic bag or plastic container with proper label and store in a cooler while on board.

      *Treatment of Samples after collection
          1. Choose fresh shellfish samples. Shellfish must be fresh.
          2. Thoroughly clean the shellfish. Remove barnacles, dirt, mud, and other foreign materials from the shellfish
          3. Remove the shellfish tissue. Carefully open the shell by cutting the adductor mucles using a knife.
          4. Drain excess water from the shellfish tissue. Drain and discard excess water from the shellfish sample.
          5. Collect about 100g of shellfish tissue.
          6. Storage of shellfish tissue samples. Put the shellfish tissue in a plastic ice. Properly label the samples with the information: English
              name, Source/Origin, Date of         
              Collection
          7. Keep the samples ina cooler/styro box with ice for transport via Victory Liner bus to be sending at BFAR-Central Office, Quezon City.
     
      *Plankton Collection

          1. Determine the depth of the sampling station
          2. Tie the plankton net with a calibrated rope (1 meter interval) and tie a 1-kg weight to the narrow end of the net to make it sink in the
              water.
          3. Lower the plankton net to the desired with the at least 1-meter clearance from the bucket (cod end) and sea bottom.
          4. Raise and wash the net and its cod-end several times by shaking the net in the water.
          5. Transfer the concentrated samples in a 200-ml polyethylene bottle and keep in the under cool condition or cooler.